ROCHE RNase, DNase-free, from bovine pancreas

Specific Activity: ≥30 units/mg protein

Storage: −20°C

UNSPSC Code: 41105600

RIDADR: NONH for all modes of transport

Application:
RNase, DNase-free, efficiently removes contaminating RNA from plasmid or genomic DNA preparations.

General description:
Pyrimidine-specific endoribonuclease that acts on single-stranded RNA. RNase, DNase-free, is a heterogeneous mixture of ribonucleases that has been prepared free of deoxyribonuclease activity according to the current Quality Control procedures. RNase, DNase-free, is particularly well suited for use in DNA isolation procedures. Before use, most RNase preparations must be boiled to remove DNase activity. This preparation of RNase does not need to be boiled; it can be used directly from the vial.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Physical form:
Solution, 500 μg/ml, in 10 mM Tris-HCl, 5 mM CaCl₂, 50% glycerol (pH 7.0).

Preparation Note:
Working concentration: The optimal working concentration for RNase, DNase free, is 2 to 5 μg/ml. The reaction volume will vary for different applications. Some suggested guidelines are given below:

1. For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl.
   • To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
   • To isolate genomic DNA from cultured mammalian cells (5 x 10⁷ cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.