Synonyms: rnase h
Specific Activity: ~40000 units/mg protein
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Features and Benefits:
• Eliminate potential sources of PCR errors.
• Increase accessibility of primers during subsequent PCR.
Application:
Ribonuclease H (RNase H) has been used for:
• In vivo RNA-primed initiation of DNA synthesis
• Elimination of mRNA during second-strand cDNA synthesis
• Site-specific cleavage of RNA
• Detection of RNA:DNA regions in double-stranded DNA of natural origin
• Removal of poly (A) sequences of mRNA if oligo (dT) is present
• RT-PCR
General description:
Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.
Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
Other Notes:
For life science research only. Not for use in diagnostic procedures. Using RNase H after the cDNA synthesis step can increase the sensitivity of a two-step RT-PCR assay.
Quality:
Absence of endonucleases, nicking activities, and ribonucleases.
Preparation Note:
Activator: The enzyme has its maximal activity in presence of SH-reagents