Synonyms: cloning of dna fragments; dna ligase; ligases
Storage: −20°C
UNSPSC Code: 12352200
Components: rAPid Alkaline Phosphatase Buffer 10x concentrated; rAPid Alkaline Phosphatase 1 U/μl; DNA Dilution Buffer 5x concentrated; T4 DNA Ligation Buffer 2x concentrated; T4 DNA Ligase 5 U/μl
RIDADR: NONH for all modes of transport
Application:
The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
• Cloning of fragments into either plasmid or phage vectors
• Linker ligation
• Recircularization of linear vector DNA
• Generation of libraries
General description:
The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Specificity:
Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.