ROCHE Pyruvate Kinase (PK), from rabbit muscle

Specific Activity: ~200 units/mg protein (At 25 °C with PEP as the substrate.)

UNSPSC Code: 12352204

RIDADR: NONH for all modes of transport

Application:
Pyruvate Kinase (PK) has been used in the kinase assay.

General description:
ATP:pyruvate 2-O-phosphotransferase

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Quality:
Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.

Physical form:
Solution in 50% glycerol (v/v), pH approximately 6

Preparation Note:
Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.

Specificity:
Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.