Synonyms: β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate; β-NADPH; 2′-NADPH hydrate; Coenzyme II reduced tetrasodium salt; Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt; NADPH Na4; TPNH2 Na4; Triphosphopyridine nucleotide reduced tetrasodium salt
Empirical Formula (Hill Notation): C21H26N7Na4O17P3 ¬? xH2O
Formula Weight: 833.35 (anhydrous basis)
Linear Formula: C21H26N7Na4O17P3 ¬? xH2O
MDL Number: MFCD00036263
Purity: ‚≥97% (dry weight)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Analysis Note:
Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm
Application:
NADPH-RO has been used for the determination of thioredoxin reductase activity.
Biochem/physiol Actions:
NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.
General description:
• Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm
• Absorbance maxima: 260 nm and 340 nm
• Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4
• pH value in water: 8.0 ± 1.0 (10 mg/ml)
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
• Purity: =97% ß-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm)
• Organic solvents: =3% (GC)
Preparation Note:
Storage conditions (working solution): 2 to 8 °C
10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.