Synonyms: β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate; β-DPNH; β-NADH; DPNH; Diphosphopyridine nucleotide, reduced form; NADH
Empirical Formula (Hill Notation): C21H27N7Na2O14P2 ¬? xH2O
Formula Weight: 709.40 (anhydrous basis)
Linear Formula: C21H27N7Na2O14P2 ¬? xH2O
MDL Number: MFCD03791273
Purity: 0.98
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Application:
NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.
Biochem/physiol Actions:
NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: 98% β-NADH-Na2 based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A260 nm-A340 nm: <2.40
Organic solvents: <4% (GC)
Specifications:
Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at 334 nm (Hg), 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at 365 nm (Hg)
Formula: C21H27N7O14P2Na2
pH value in water: 7.5 ± 0.5 (100 mg/ml)
Physical form:
Amorphous powder
Preparation Note:
Activator: Mg2+ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C