Synonyms: β-Nicotinamide adenine dinucleotide hydrate; β-DPN; β-NAD; Coenzyme 1; Cozymase; DPN; Diphosphopyridine nucleotide; NAD; Nadide
Empirical Formula (Hill Notation): C21H27N7O14P2 ¬? xH2O
Formula Weight: 663.43 (anhydrous basis)
Linear Formula: C21H27N7O14P2 ¬? xH2O
MDL Number: MFCD00036253
Purity: 98% (dry weight)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Analysis Note:
Absorbance coefficients: 17.6 x 103 l x mol-1 x cm-1 at 260 nm, 5.9 x 103 l x mol-1 x cm-1 at 333 nm (CN complex)
Biochem/physiol Actions:
Electron acceptor
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Absence of contaminants: LDH inhibitors are not detectable.
Organic solvents: <0.5% (GC)
Preparation Note:
Storage conditions (working solution): An aqueous solution in a concentration of 10 mg/ml (pH 2.5 to 3) maintains > 99% of its NAD content after 8 weeks of storage at 2 to 8 °C.
The stability of an aqueous solution in a concentration of 40 mg/ml is not expected to differ from that of a solution in a concentration of 10 mg/ml.
An aqueous solution in a concentration of 50 mg/ml (pH 2.0) maintains 97% of its NAD content after 2 days, 94% after 6 days and 91% after 10 days of storage at 2 to 8 °C and about 83% after 10 days of storage at 33 °C.
An aqueous solution in a concentration of 1.38 mg/ml shows a decrease of NAD content of 4% after 8 weeks of storage at 2 to 8 °C, stored at -15 to -25 °C the stability is expected to be > 6 months. A solution of NAD in triethanolamine buffer (pH 5.0) maintians 90% of its NAD content after
10 days of storage at 2 to 8 °C and 80% after 10 days of storage at 33 °C.