Synonyms: Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
MDL Number: MFCD00081739
Purity: ‚≥90% (SDS-PAGE)
Storage: −20°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Application:
Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
General description:
Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
Physical form:
Solution, filtered through 0.2 ?m pore size membrane
Preparation Note:
Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
Sequence:
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.