Synonyms: Isopropyl β-D-1-thiogalactopyranoside; iptg; IPTG; Isopropyl β-D-thiogalactoside
Empirical Formula (Hill Notation): C9H18O5S
Formula Weight: 238.3
Linear Formula: C9H18O5S
MDL Number: MFCD00063273
Storage: 2-8°C
Beilstein Registry Number: 4631
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
General description:
Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes:
The amount of labeled DNA depends on:
• amount of template DNA
• purity of template DNA
• conformation of template DNA
• average size of labeled fragment
Specifications:
Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Preparation Note:
Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long