Purity: >95% (SDS-PAGE)
Storage: −20°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Application:
Recombinant murine IL-2 is produced in E. coli and exhibits the following:
• Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
• Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
• Weakly inhibits the binding to human responder cells.
• Shares identical biological and immunological activities with human IL-2.
• Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
• used for the stimulation of murine T-cells
General description:
Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Preparation Note:
Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.
Sequence:
One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.
Specificity:
Mouse IL-2 is effective on mouse cells, but not on human cells.