Purity: >98% (SDS-PAGE)
Specific Activity: >26 units/mg protein (At least the same specific activity (EC<sub>50</sub>) compared to the indicated standard is guaranteed.)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Insulin shows a broad range of activities on a variety of somatic cells. Recombinant human insulin can be used to stimulate growth and proliferation of cultured cells and to investigate insulin activity on sensitive cells used in research studies. It is also a component of serum-free media formulations for most primary cells and cell lines.
General description:
Recombinant Insulin, human, is produced in yeast and purified by standard chromatographic techniques. Insulin regulates blood glucose. In muscles and adipocytes it stimulates glucose intake and metabolism. It also controls the expression and various enzymes.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Endotoxin level: <0.1EU/μg (LAL-test)
Note: 1 EU corresponds to 0.1ng
Physical form:
Lyophilizate from a hydrochloric acid solution (pH 2.3) (crystalline insulin)
Preparation Note:
Working concentration: 1-10 μg/ml
Recommended concentration for serum-free cell culture is 1-10 μg/ml.
The concentration of insulin required for stimulation of cell growth in almost all cases is extraordinarily high compared with the physiological concentration. Insulin may be mimicking insulin-like growth factors (IGFs, somatomedins) for some cell lines, and high insulin concentrations may be necessary to occupy receptors which have a high affinity for IGFs and a lower affinity for insulin.
Working solution: Dissolve Insulin, human, recombinant (100 mg or 500 mg), in sterile, double-dist. water (final concentration: 10 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The reconstituted, undiluted solution is stable at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution:
Reconstitute in sterile double-distilled water (final concentration 10 mg/ml), further dilution with PBS (phosphate buffered saline) or medium containing 1 mg/ml (0.1%) BSA (bovine serum albumin) [or HSA (human serum albumin)], or 1 to 10% serum.
Sequence:
Two polypeptide chains (A-chain: 21 amino acids, B-chain: 30 amino acids, connected by two disulfide bridges) identical to natural, human insulin.
Specificity:
Insulin, human, is active on most mammalian cells.