Storage: −20°C
UNSPSC Code: 12352200
Components: Enzyme Solution (TdT); Label Solution (fluorescein-dUTP)
RIDADR: UN 1556 6.1 / PGIII
Features and Benefits:
• Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling
• Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction
• Convenient: No secondary detection system required
• Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis
Application:
The In Situ Cell Death Detection Kit, Fluorescein, is a precise, fast, and simple nonradioactive technique to detect and quantify apoptotic cell death at the single-cell level by fluorescence microscopy and quantitative detection by flow cytometry in cells and tissues. Thus, the In Situ Cell Death Detection Kit can be used in many different assay systems.
Examples are:
• Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research
• Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research
• Typing of cells undergoing cell death in heterogeneous populations by double staining procedures
General description:
Kit for the detection and quantification of apoptosis at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology); analysis by fluorescence microscopy or flow cytometry.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working solution: Add total volume (50 μl) of Enzyme Solution to the remaining 450 μl Label Solution to obtain 500 μl TUNEL reaction mixture.
Mix well to equilibrate components.
Specificity:
The TUNEL test kit reaction preferentially labels DNA strand breaks generated during apoptosis. This allows discrimination of apoptosis from necrosis and from primary DNA strand breaks induced by cytostatic drugs or irradiation.