Synonyms: dna, human; homo sapiens dna
Storage: 2-8°C
UNSPSC Code: 41106310
RIDADR: NONH for all modes of transport
Application:
This DNA is suitable for Southern hybridization analysis, genomic library construction, and the amplification of large DNA targets by the Expand System. It has been used to assess the quality or integrity of DNA sample using qPCR or real time (RT) PCR, and as a control during DNA sequencing.
The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.
General description:
High molecular weight (>50kb) genomic DNA isolated from human blood (buffy coat) by the method of Sambrook et al..
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Molecular weight: The preparation is electrophoretically separated on a 0.5% agarose gel and the gel is stained with ethidium bromide. The molecular weight of the purified genomic DNA is greater than 50kb.
Function test: The preparation is used as template in a PCR with the Expand PCR System and appropriate primers from the human tPA Control Primer Set. Amplification products up to 27kb long are obtained.
Absence of contaminating organisms: The serum used for this preparation was tested for HBs antigen and the presence of antibodies to HIV-1, HIV-2, HCV. All tests were negative.
Physical form:
Solution in 10 mM Tris HCl, 1 mM EDTA, pH 8.0