Specific Activity: ~450 units/mg protein (At 25 °C and pH 7.6 with D-glucose and ATP as the substrates.)
Storage: 2-8°C
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Application:
Use Hexokinase for the determination of D-glucose, D-fructose, and D-sorbitol in food or biological research samples. Hexokinase may also be used for the assay of other saccharides, which are convertible to glucose or fructose, and is, therefore, useful in the assay of many glycosides.
Hexokinase has been used in the enzymatic detection of nitric oxide and in the measurement of ATP by luminescence method.
Biochem/physiol Actions:
Hexokinase catalyzes the conversion of hexoses to hexose-6-phosphate in an ATP-dependent manner. It is also called as the glycolytic enzyme as it catalyzes the first step in glucose metabolism with the formation of glucose-6-phosphate (G6P). In eukaryotes, it functions as a glucose sensor.
General description:
ATP:D-hexose 6-phosphotransferase
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Contaminants: <0.002% PGI, <0.0005% G6P-DH and GR, each, <0.001% CK, MK, 6-PGDH, and ADH, each, <0.05% GIDH and invertase (β-fructosidase), each, <30 μg/ml glucose (enzymatically)
Physical form:
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.5
Preparation Note:
Activator: Hexokinase requires Mg2+ ions (Km = 2.6 mM) for activity. Catecholamines too increase activity.
Stabilizers: Thiols