Specific Activity: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Analysis Note:
Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)
Application:
It was used in enzymatic determination of C6 phosphorylation of glycogen.
General description:
Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH
Physical form:
Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6
Preparation Note:
Activator: HCO3- (≤ 0.3 M) activates slightly.
Specificity: