Synonyms: G 418 disulfate salt solution; O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt; neomycin; Antibiotic G418
Empirical Formula (Hill Notation): C20H40N4O10 ¬? 2H2SO4
Formula Weight: 692.71
Linear Formula: C20H40N4O10 ¬? 2H2SO4
MDL Number: MFCD00058314
Purity: ‚≥98% (TLC)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
• Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
• Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
• Function tested in a cell culture assay - ensures effectiveness.
• Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
• Cost effective, since the selected colonies are stable.
Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane
Application:
G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neor) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.
Biochem/physiol Actions:
Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.
Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
General description:
O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: = 98%, as shown by TLC.
Function testing: in cell culture.
Preparation Note:
Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).