Specific Activity: ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Most widely used in cofactor recycling systems for NADH.
General description:
Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Contaminants: <0.005% LDH and ADH each, <0.1% MDH
Specificity:
Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.