Synonyms: Fluorescein-12-UTP tetralithium salt; Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
Empirical Formula (Hill Notation): C39H41N4O22P3
Formula Weight: 1010.68
Linear Formula: C39H41N4O22P3
Storage: −20°C
UNSPSC Code: 41116100
RIDADR: NONH for all modes of transport
Application:
Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
General description:
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.
Other Notes:
For life science research only. Not for use in diagnostic procedures.