UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
• Increase qPCR sensitivity and specificity.
Produce lower cycle threshold (Ct) values.
• Use the master mix with any probe-based assay.
Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe.
• Amplify and detect a broad range of DNA or cDNA targets.
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.
• Visualize amplification products on agarose gels.
• Use robotic pipetting stations to set up qPCR reactions.
Use a master mix that is stable at room temperature during extended reaction setup times.
• Prevent false positives resulting from carryover contamination.
Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.
Application:
FastStart Universal Probe Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
General description:
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).
Other Notes:
Article (PDF, 597.72 KB)
Quality:
Function test: Each lot is tested for performance in qPCR using three templates: a GC–rich template, an AT-rich template, and a long template (approximately 440 bp).
Components:
FastStart Universal Probe Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and a reference dye.
Legal Information:
LightCycler is a registered trademark of Roche