UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
• Improve PCR sensitivity and specificity:
Rely on this mix′s FastStart Taq DNA Polymerase to minimize the formation of nonspecific amplification products through hot start PCR.
• Avoid over-estimation of qPCR results:
Eliminate nonspecific amplification products and primer-dimers that would increase the amount of bound quantified SYBR Green I.
• Amplify and detect a broad range of DNA or cDNA targets:
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich. Works with any real-time PCR instrument other than the LightCycler® Instruments.
• Use any real-time PCR instrument other than the LightCycler® Instruments:
Choose from two formulations - one that contains the ROX reference dye and one without ROX.
• Save time and effort in qPCR preparation:
Rely on this easy-to-use 2x master mix to eliminate the need to mix components, titrate MgCl2, or perform other time-consuming optimization steps.
• Prevent false positives resulting from carryover contamination:
The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.
Application:
The FastStart SYBR Green Master is a ready-to-use, 2x concentrated master mix that contains all the reagents (except primers and template) needed for running quantitative, real-time DNA detection assays, including qPCR and two-step qRT-PCR, in the SYBR Green I detection format. Use the FastStart SYBR Green Master with any real-time qPCR instrument other than the LightCycler® Instruments.
General description:
The FastStart SYBR Green Master is a ready-to-use reagent mix that simplifies the preparation of reactions for qPCR and two-step qRT-PCR using the intercalating dye SYBR Green I for DNA detection and analysis. In combination with a real-time PCR instrument and suitable PCR primers, FastStart SYBR Green Master allows very sensitive detection and quantification of defined DNA sequences.
Do not use this product with the LightCycler® Instruments.
In principle, the FastStart SYBR Green Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, you would need to adapt your detection protocol to the reaction conditions of the particular real-time PCR instrument used and design specific PCR primers for each target. See the Operator′s Manual of your real-time PCR instrument for general recommendations.
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step qRT-PCR. Insist on the Transcriptor First Strand cDNA Synthesis Kit - designed and function-tested for qPCR, and efficient with all real-time PCR instruments.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (about 440 bp).
Components:
FastStart SYBR Green Master, 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and SYBR Green I.
Legal Information:
LightCycler is a registered trademark of Roche