Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
The FastStart Master has modified recombinant FastStart Taq DNA Polymerase, inactive at temperatures below +75°C. The kit also contains an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates.
• Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier.
• Use robotic setup.
Use this enyzme mix stable for 24 hours at +15 to +25°C.
• Reduce Setup time.
Store master mixes at +2 to +8°C for immediate use for up to one month.
Contents
2x concentrated master mix containing FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP, dTTP).
Application:
• The FastStart™ PCR Master is a convenient alternative to the single reagents for nearly all hot start PCR applications. It can be applied for the amplification of genomic DNA and cDNA targets up to 2 kb long, or longer fragments on plasmid DNA targets, with high specificity, sensitivity, and yield. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. It can be used for:
• Hot Start PCR up to 2 kb
• Hot Start RT-PCR up to 2 kb
• High-throughput PCR
• Direct colony PCR
General description:
Hot start PCR is effortless using the FastStart PCR Master, a ready-to-use, double-concentrated hot start master mix. This master mix contains FastStart Taq DNA Polymerase, Roche high quality PCR-Grade Nucleotides, and all other reagents, except PCR primers and template, required for PCR and two-step RT-PCR on thermal cycler instruments.
FastStart PCR Master accepts modified nucleotides such as DIG-dUTP. Labeling with DIG-dUTP can be achieved by adding the modified nucleotide in a final concentration of 10 μM to the final volume.
Combine this master mix with Roche ′s Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step RT-PCR.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Legal Information:
FastStart is a trademark of Roche