Synonyms: long template pcr; longe range pcr; nucleotide mixes and sets; pcr enzymes
Storage: −20°C
UNSPSC Code: 12352200
Components: Expand Long Range Enzyme Mix; Expand Long Range Buffer with 12.5 mM MgCl2 5x concentrated; Expand Long Range Buffer without MgCl2 5x concentrated; MgCl2 Stock Solution 25 mM; DMSO 100%; PCR Nucleotide Mix
RIDADR: NONH for all modes of transport
Features and Benefits:
Expand Long Range PCR System is ideal for genome mapping and sequencing, contig construction, characterization of cloned sequences in lambda phages or cosmids, and eukaryotic-gene cloning and analysis.
Amplify DNA fragments from 5 to 20kb from human genomic DNA, and 40kb from λDNA using supplied reaction buffers.
Use 3.75U for a standard 50μl PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
• Amplify long templates
up to 25kb with high specificity, yield, and increased fidelity.
• Save time and resources.
Use one buffer, and fine-tune using the DMSO and MgCl2 solution.
• Use this kit for all long template applications.
Obtain high yields of your desired fragment.
• Cost-effective.
Use the convenient premixed solution of PCR grade dNTPs.
Application:
The Expand Long Range dNTPack is optimized to efficiently amplify large genomic DNA fragments from 5kb to 25kb in combination with a threefold higher fidelity than Taq DNA Polymerase. The Expand Long Range dNTPack is ideally suited for:
• Genome mapping and sequencing
• Contig construction
• Characterization of cloned sequences in lambda phages or cosmids
• Cloning and analysis of eukaryotic genes
• Rapid identification and cloning of complete genes from genomic DNA using cDNA sequence as starting material
• Two-step RT-PCR
Use the specially designed buffer set, ultrapure PCR Nucleotide Mix, and optimized blend of thermostable DNA polymerases for:
• PCR
• Large-fragment amplification
• RT-PCR
For amplicon sizes of 20 to 35kb choose Expand 20kb PLUS PCR System, dNTPack.
General description:
The Expand Long Range dNTPack is the second-generation version of the Expand Long Template PCR System, optimized and designed to consistently amplify PCR products from 5kb up to 25kb from genomic DNA (or up to 40kb from λ DNA templates). The supplied buffer can be used for all PCR product sizes. In addition, a buffer without MgCl2, and separate vials of MgCl2 and DMSO are included if optimization is needed. Expand Long Range dNTPack contains a special enzyme mix that consists of thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of PCR product. Due to the inherent 3′–5′ exonuclease activity of the proofreading polymerase, Expand Long Range dNTPack copies DNA three times more accurately than Taq DNA Polymerase.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Each lot is PCR function-tested using human genomic DNA and specific primers for a 9kb, 12kb, and 15kb fragment. The enzyme mix is tested for the absence of any contaminating activities, including endo- or exonucleases and nicking activity according to the current Quality Control procedures.
Preparation Note:
Working concentration: Standard enzyme concentration: 3.5 U per 50 μl reaction
Specificity:
Heat inactivation: > 92 °C
Legal Information:
Expand is a trademark of Roche