Storage: −20°C
UNSPSC Code: 41105335
RIDADR: NONH for all modes of transport
Application:
DNA Molecular Weight Marker XIII (50 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest, followed by separation on agarose gels. DNA Molecular Weight Marker XIII (50 bp ladder) can be used in conjunction with DNA Molecular Weight Marker XIV (100 bp ladder) for precise size determination.
The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
General description:
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 50 to 750 bp and an additional band of 2642 bp
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Physical form:
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note:
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Sequence:
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, and an additional band of 2642 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.