Storage: −20°C
UNSPSC Code: 41105335
RIDADR: NONH for all modes of transport
Features and Benefits:
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application:
DNA Molecular Weight Marker III, DIG-labeled, is used as a size standard in Southern blot analysis when the DIG System for Nucleic Acid Labeling and Detection is used.
General description:
DNA Molecular Weight Marker III, DIG-labeled is a fragment mixture prepared by cleavage of λDNA with EcoR I and Hind III. The size ranges from 0.12 to 21.2kbp.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Sequence:
The mixture contains 13 fragments wit the following base pair lengths: 125, 564, 831, 947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, and 21226 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.