Storage: −20°C
UNSPSC Code: 41106310
RIDADR: NONH for all modes of transport
Application:
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
General description:
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Physical form:
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Preparation Note:
Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
Sequence:
Chain Length 120 to 3,000 nucleotides