Synonyms: lambda dna
UNSPSC Code: 41106310
RIDADR: NONH for all modes of transport
Application:
λDNA is used as one of the standard substrates in restriction enzyme research. It has been used for DNA overstretching measurements using confocal fluorescence microscopy.
General description:
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5’ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Physical form:
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Sequence:
Chain Length 48,502 bp