ROCHE Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

Synonyms: Digoxigenin NHS-ester; dig-nhs-ester; ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester; DIG NHS

Empirical Formula (Hill Notation): C₃₅H₅₀N₂O₁₀

Formula Weight: 658.78

Linear Formula: C₃₅H₅₀N₂O₁₀

MDL Number: MFCD02093394

Purity: >90% (elementary analysis)

Storage: −20°C

UNSPSC Code: 41105600

RIDADR: UN 2811 6.1 / PGI

Application:
The preparation is used for DIG-labeling of:
• proteins
• 5′-amino-substituted oligonucleotides via amino groups (under gentle conditions)
• oligonucleotide sequence

General description:
Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Preparation Note:
Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Principle:
The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.