Purity: ‚≥85% (HPLC)
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Digoxigenin-11-dUTP, alkali-labile, is for nonradioactive DNA labeling (e.g., random-primed or nick translation). The alkali-labile compound should be used for labeling of probes which are preferentially used in hybridization experiments where stripping and reprobing of the membrane is intended. DIG-11-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, Terminal Transferase and reverse transcriptase.
• Labeled DNA can be subsequently detected with the: DIG Nucleic Acid Detection Kit
• DIG Luminescent Detection Kit
• Fluorescence in situ hybridization (FISH).
Note: Do not use in experiments where alkaline treatment is required. For this application, use the alkali-stable DIG-11-dUTP.
General description:
Digoxigenin-11-dUTP is alkali-labile as a 1mM solution of the tetralithium salt. Digoxigenin is bound to deoxyuridine triphosphate via an alkali-sensitive ester linkage.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.