UNSPSC Code: 12352200
Components: Reaction Buffer 5x concentrated; CoCl<SUB>2</SUB> Solution 25 mM; DIG-dUTP Solution 1 mM; dATP Solution 10 mM; Recombinant Terminal Transferase 400 U/μl; Control Oligonucleotide, unlabeled 20 pmol/μl; Oligonucleotide, DIG-dUTP/dATP tailed 2.5 pmol/μl; Control DNA 0.25 mg/ml; Glycogen Solution 20 mg/ml; DNA Dilution Buffer, 50 μg/ml fish sperm DNA; Poly(A) Solution 10 mg/ml
RIDADR: UN 3316 9
Features and Benefits:
Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).
• Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
• Fast hybridization kinetics, due to the small size of oligonucleotides
• Single-stranded probes, no renaturation during hybridization
• Sequence can be designed according to the experiment
• Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells
General description:
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working concentration: Oligonucleotides
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.
Principle:
DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).