Storage: −20°C
UNSPSC Code: 12352200
Components: DAB/metal concentrate, 25 ml 10x concentrated; Peroxide Buffer, 250 ml
RIDADR: UN1230 - class 3 - PG 2 - Methanol, solution
Application:
• DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
• In situ hybridization
• Western blot
• Dot blot
General description:
The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working solution:
- Determine the volume of substrate required for the development of peroxidase.
• Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
Note: Do not bring to 15 to 25 °C.
• Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 μl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
• Mix well.
• Add the 1x working solution to the tissue or blot until desired substrate development.
Note: The 1x solution is stable over many hours.
For best results, store the working solution at 2 to 8 °C when not using.
Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.