Specific Activity: ≥0.25 U/mg (At 25 °C with citrate as the substrate.)
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Application:
It was used in enzymatic assay to measure citrate concentration using microtiter plates.
General description:
Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each
Physical form:
Lyophilizate, stabilized with BSA, sucrose, MgSO4 and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.
Preparation Note:
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)