Purity: ‚≥90%
Specific Activity: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.
General description:
Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.
Preparation Note:
Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.
Reconstitution:
Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl2, pH 7.8).
Specificity:
Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.