Synonyms: hybridoma cloning supplement
Storage: −20°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Analysis Note:
Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.
Features and Benefits:
Contents
Solution in RPMI 1640, filtered through 0.2μm pore size membrane
Application:
BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
General description:
BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Cell Line Origin:
BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.
Physical form:
Solution in RPMI 1640, filtered through 0.2 μm pore size membrane
Preparation Note:
Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.