Storage: −20°C
UNSPSC Code: 41116100
RIDADR: NONH for all modes of transport
Application:
Biotin-labeled RNA can be used as a hybridization probe for:
• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization
General description:
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively. The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Physical form:
Biotin-16-UTP, tetralithium salt, 10mM solution