Synonyms: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP® p-toluidine salt; X-phosphate p-toluidine salt
Empirical Formula (Hill Notation): C8H6BrClNO4P ¬? C7H9N
Formula Weight: 433.62
Linear Formula: C8H6BrClNO4P ¬? C7H9N
MDL Number: MFCD00040636
Purity: 99% (HPLC)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Analysis Note:
Absorption: Absorption maximum in the visible light is not determined.
Application:
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
General description:
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Performance test: incubation with AP
Substrates:
Histochemical substrate for alkaline phosphatase.
Legal Information:
BCIP is a registered trademark of Sigma-Aldrich Co. LLC