Purity: >95% (HPLC)
Storage: −20°C
UNSPSC Code: 12352204
RIDADR: UN 2265 3 / PGIII
Application:
Use BCIP in alkaline phosphatase detection:
• Southern blot
• Northern blot
• Western blot
• Colony and plaque hybridization
• In situ hybridization
General description:
BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function test: incubation with AP
Physical form:
Solution (50 mg/ml) in dimethylformamide (DMF)
Principle:
Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.