Synonyms: metal chelate affinity chromatography
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Anti-His6 is used for the detection of His6-tagged proteins in:
• Immunoblotting (e.g., dot blots and western blots)
• Immunoprecipitation
• Immunoassays (ELISA)
• Immunocytochemistry
• Immunofluorescence
General description:
Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
• ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
• Immunoprecipitation: 0.5 to 5 μg/ml
• Western blot: 0.2 to 0.5 μg/ml
Reconstitution:
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Specificity:
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.