Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:
• Dot blots
• ELISA
• Immunocytochemistry
• Immunoprecipitation
• Western blots
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.
General description:
Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Function tested in western blot.
Immunogen:
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Preparation Note:
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
• ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
• Immunoprecipitation: 0.5 to 5 μg/ml
• Western blot: 50 to 200 ng/ml
Reconstitution:
Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.
Specificity:
Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).