Purity: >90% (HPLC and SDS-PAGE)
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
• Dot blots
• Immunochemistry
• Immunoprecipitation
• Western blotting
Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.
Immunogen:
Amino acids 98-106 from the human influenza virus hemagglutinin protein.
Preparation Note:
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
• Dot blot: 0.1 to 1 μg/ml
• Immunfluorescence: 1-10 μg/ml
• Western blot: 0.1 to 1 μg/ml
Reconstitution:
11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.
Specificity:
Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).