Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
Contents
Solution, stabilized
Application:
Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
• Dot blot
• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Southern blot
• Western blot
• Electrochemical biosensing technique for the detection of 16S rRNA
Biochem/physiol Actions:
Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).
General description:
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
• Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
• ELISA: 150 to 300mU/ml
• Immunohistocytochemistry: 250 to 500mU/ml
• In situ hybridization: 1.5 to 7.5U/ml
• Southern blot: 150mU/ml
• Western blot: 250 to 500 mU/ml
Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Specificity:
The polyclonal antibody reacts with free and bound fluorescein.