Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Use Anti-Digoxigenin-POD (poly), Fab fragments for the detection of digoxigenin-labeled compounds using:
• Dot blot
• ELISA
• Southern blot
• Western blot
• In-situ hybridization
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
General description:
Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 50 to 100 mU/ml
• ELISA: 2 to 50 mU/ml
• Western blot: 50 to 100 mU/ml
• Southern blot: 50 to 100 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Reconstitution:
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Specificity:
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.