Purity: ‚≥95% (HPLC)
Specific Activity: ≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.
General description:
The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Specifications:
≥7,000 U/mg (+37°C; pNPP; DEA)
Physical form:
Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.
Preparation Note:
Activator: Mg2-, Co2- and Mn2-ions
Specificity:
Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.