Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3'-pyrimidinooligonucleotidohydrolase
CAS Number 9001-99-4
EC Number 232-646-6
Enzyme Commission (EC) Number 3.1.27.5
MDL number MFCD00132181
Components
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
Analysis Note
Protein determined by E1%/280.
Protein determined by E1%/280.
Features and Benefits
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
General description
RNase A is an endoribonuclease that attacks at the 3'OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
Other Notes
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
Application
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Suitable for:
• RNase protection assays
• Removal of unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolysis of RNA contained in protein samples
• Plasmid DNA purification