Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3'-pyrimidinooligonucleotidohydrolase
CAS Number: 9001-99-4
Enzyme Commission (EC) Number: 3.1.27.5
Components
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
Analysis Note
Protein determined by E1%/280.
Application
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Features and Benefits
- RNase protection assays
- Remove unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolyze RNA contained in protein samples
- Purification of DNA
RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
Grade: For molecular biology
Form: (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Mol. Wt.: Mol. wt. 13.7 kDa; Mol. wt. ~13,700
Concentration: 1400 - 2800 U/mL
Foreign activity: Endonuclease and exonuclease, none detected; NICKase and DNase, none detected
Shipped in: Dry Ice
Storage temp.: -20°C