Reverse Transcriptase (RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. This RT is an RNA-directed DNA polymerase which lacks 3´→ 5´ exonuclease activity and can produce full-length cDNA from long RNA templates.
Product Source:
- The gene encoding recombinant MMLV Reverse Transcriptase with mutated RNase H domain is expressed in E. coli.
Product Includes:
- Reverse Transcriptase
- 10X RT Reaction Buffer
Applications:
- First strand cDNA synthesis
- Gene expression analysis
Benefits:
- Recombinant RT with greatly reduced RNase H activity.
- Active at temperatures up to 55°C.
- Highly efficient at producing full-length cDNA from low concentration of RNA.
Unit Definition:
One unit is defined as the amount of enzyme required to incorporate 1nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
10X RT reaction buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C
Storage Temperature: – 20ºC
Heat Inactivation: 65°C for 20 min