The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6xHis tag at the C-terminus.
Synonyms: S glycoprotein Subunit1; S1 protein; Spike glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein
Purity: >95% (SDS-PAGE)
Storage: -20C
Biochem Physiol Actions: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2mug/mL (100 muL/well) can bind Recombinant SARS-COV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay.