Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.
Synonyms: 2019-nCoV RBD Protein; NCP-CoV RBD Protein; S glycoprotein Subunit1 RBD Protein; S1-RBD protein; novel coronavirus RBD Protein
Purity: >95% (HPLC); >95% (SDS-PAGE)
Storage: -20C
Biochem Physiol Actions: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2mug/mL (100 muL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.