KE20014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Rat VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous rat VEGF. The assay recognizes rat VEGF. An antibody specific for rat VEGF has been pre-coated onto the microwells. The rat VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat VEGF is added to detect the captured rat VEGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.