KE20011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP1 ELISA kit is to be used to detect and quantify protein levels of endogenous FABP1. The assay recognizes rat FABP1. An antibody specific for FABP1 has been pre-coated onto the microwells. The FABP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat FABP1 is added to detect the captured rat FABP1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.