For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.
- Safe. Reagents are non-toxic.
- Linear detection range 0.9 - 200 mg /dL hemoglobin.
- The "mix-and-read" 5 minute procedure.
- Can be readily automated to process tens of thousands of samples per day.
- Assays can be executed in microplate or cuvet.
- Method: OD400 nm.
HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration. Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and is ideal for measuring hemolysis in low hemoglobin samples (e.g. serum and plasma).